Volume 6.05 | Feb 13

Mammary Cell News 6.05 February 13, 2014
Mammary Cell News
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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TOP STORY
Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-Like Breast Cancer
Scientists report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell-like properties, and tumorigenicity of BLBC cells. [Cancer Cell] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)
LABORATORY RESEARCH

EZH2 Expands Breast Stem Cells through Activation of NOTCH1 Signaling
Researchers identify an unrecognized Enhancer of Zeste Homolog 2 (EZH2)/NOTCH1 axis that controls breast tumor-initiating cells in triple-negative breast carcinomas. EZH2 overexpression increases NOTCH1 expression and signaling, and inhibition of NOTCH1 activity prevents EZH2-mediated stem cell expansion in nontumorigenic breast cells. [Proc Natl Acad Sci USA] Abstract

ß1 Integrin Inhibition Elicits a Prometastatic Switch through the TGFß-miR-200-ZEB Network in E-Cadherin-Positive Triple-Negative Breast Cancer
Researchers found that the loss of ß1 integrins attenuated breast tumor growth but markedly enhanced tumor cell dissemination to the lungs. When cultured in three-dimensional extracellular matrix scaffolds, antibodies that blocked ß1 integrin function or knockdown of ß1 switched the migratory behavior of human and mouse E-cadherin-positive triple-negative breast cancer cells from collective to single cell movement. [Sci Signal] Abstract

Intracellular Routing in Breast Cancer Cells of Streptavidin-Conjugated Trastuzumab Fab Fragments Linked to Biotinylated Doxorubicin-Functionalized Metal Chelating Polymers
To evaluate the cellular and nuclear uptake of trastuzumab Fab-streptavidin-biotin-metal-chelating polymer-doxorubicin (Dox), researchers incubated human epidermal growth factor receptor-2+ SK-BR-3 human breast cancer cells with the complexes saturated with stable In3+ and visualized their distribution by confocal fluorescence microscopy, monitoring the fluorescence of Dox. [Biomacromolecules]
Abstract | Graphical Abstract

Estradiol Stimulates Glucose Metabolism via 6-Phosphofructo-2-Kinase (PFKFB3)
Scientists demonstrated that PFKFB3 expression is highest in stage III lymph node metastases relative to normal breast tissues and that exposure of human MCF-7 breast cancer cells to estradiol causes a rapid increase in 14C-glucose uptake that is coincident with an induction of PFKFB3 mRNA, protein expression and the intracellular concentration of its product, fructose-2,6-bisphosphate. [J Biol Chem] Abstract

Differential ERa-Mediated Rapid Estrogenic Actions of Ginsenoside Rg1 and Estren in Human Breast Cancer MCF-7 Cells
Investigators addressed the differential abilities of Rg1 and estren in terms of the ability to activate estrogen receptor (ER) and the ability to induce ER translocation in MCF-7 cells. [J Steroid Biochem Mol Biol] Abstract

Cellular Responses of BRCA1-Defective and Triple-Negative Breast Cancer Cells and In Vitro BRCA1 Interactions Induced by Metallo-Intercalator Ruthenium(II) Complexes Containing Chloro-Substituted Phenylazopyridine
HCC1937 cells were significantly more sensitive to the ruthenium complexes than the MDA-MB-231 and MCF-7 cells. Treatment demonstrated a higher degree of cytotoxicity than cisplatin against all three cell lines. [BMC Cancer] Abstract | Full Article

GPER Functions as a Tumor Suppressor in MCF-7 and SK-BR-3 Breast Cancer Cells
The orphan, membrane-bound estrogen receptor (GPER)-specific agonist G-1 inhibited breast cancer cell proliferation in concentration-dependent manner via induction of the cell cycle arrest in M-phase, enhanced phosphorylation of histone 3 and cell apoptosis. [J Cancer Res Clin Oncol] Abstract

Human Sprouty1 Suppresses Growth, Migration, and Invasion in Human Breast Cancer Cells
Researchers demonstrated that in breast cancer cell lines, MDA-MB-231 and T47D, transfection-induced overexpression of human Sprouty1 reduced cell population, proliferation, and colony formation in vitro without affecting cell apoptosis. [Tumor Biol] Abstract

CLINICAL RESEARCH

The PAM50 Risk-of-Recurrence Score Predicts Risk for Late Distant Recurrence after Endocrine Therapy in Postmenopausal Women with Endocrine-Responsive Early Breast Cancer
Researchers assessed the prognostic value of the PAM50 risk-of-recurrence score on late distant recurrence in a large cohort of postmenopausal, endocrine-responsive breast cancer patients. [Clin Cancer Res] Abstract

A Randomized Phase III Study Comparing Pegylated Liposomal Doxorubicin with Capecitabine as First-Line Chemotherapy in Elderly Patients with Metastatic Breast Cancer: Results of the OMEGA Study of the Dutch Breast Cancer Research Group BOOG
Both pegylated liposomal doxorubicin and capecitabine demonstrated comparable efficacy and acceptable tolerance as first-line single-agent chemotherapy in elderly patients with metastatic breast cancer, even in vulnerable patients or patients aged =75 years. [Ann Oncol] Abstract

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REVIEWS
Aberrant DNA Methylation of MicroRNA Genes in Human Breast Cancer – A Critical Appraisal
The author discusses the publications describing aberrant methylation of microRNA genes in human breast cancer cells. Among the anti-tumorigenic properties of epigenetically inactivated microRNA genes, the inhibition of proliferation and of epithelial-to-mesenchymal transition are the best studied. [Cell Tissue Res] Abstract

Visit our reviews page to see a complete list of reviews in the mammary cell research field.
 
INDUSTRY NEWS
Generex Provides Update on Antigen Express Phase II AE37 Breast Cancer Vaccine Trial
Generex Biotechnology Corporation announced that recent results from a Phase II study of a novel cancer vaccine designed to prevent relapse in patients who have had breast cancer corroborate prior results from an interim analysis conducted in October of 2011. [Generex Biotechnology Corporation] Press Release

Syndax Announces NCI and FDA Reach Agreement on a Special Protocol Assessment (SPA) for the Phase III Trial of Entinostat in Patients with Advanced ER+ Breast Cancer
Syndax Pharmaceuticals, Inc. announced that the Division of Cancer Treatment and Diagnosis of the National Cancer Institute (NCI) has reached a special protocol assessment agreement with the U.S. Food and Drug Administration (FDA) for the planned pivotal Phase III trial of entinostat in patients with advanced breast cancer. [Syndax Pharmaceuticals, Inc.] Press Release

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POLICY NEWS
National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)
 
EVENTS
NEW Tumor Microenvironment and Signaling
May 7-10, 2014
Heidelberg, Germany

Visit our events page to see a complete list of events in the mammary cell community.
 
JOB OPPORTUNITIES
NEW PhD Position – Grading CIN in the Mammary Gland (European Molecular Biology Laboratory)

NEW Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

NEW Research Technologist – Particle Chemistry (STEMCELL Technologies Inc.)

NEW Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

Studentship – Microtubules and End-Binding Proteins in Epithelial Remodeling and Breast Cancer Invasion (University of East Anglia)

Postdoctoral Fellow – Mechanisms that Contribute to Metastases of Breast Cancer Cells (Mount Sinai Medical Center/Icahn School of Medicine at Mount Sinai)

Postdoctoral Fellow – Hodgkin Lymphoma and Breast Cancer (Hackensack University Medical Center)

Postdoctoral Position – Functions of Epigenetic Enzymes in Breast Cancer (University of Wisconsin-Madison)

Postdoctoral Position – Breast Cancer Metastasis (Weizmann Institute of Science)

Postdoctoral Fellow – Morphogenetic Signaling Pathways in Development and Disease (University of Rochester Medical Center)

Postdoctoral Research Fellow (Brigham & Women’s Hospital and Harvard Medical School)

Postdoctoral Position – Stem Cells and Cancer (Brigham & Women’s Hospital – Harvard Medical School)


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