Volume 6.05 | Feb 13

Mammary Cell News 6.05 February 13, 2014
Mammary Cell News
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Disrupting the Interaction of BRD4 with Diacetylated Twist Suppresses Tumorigenesis in Basal-Like Breast Cancer
Scientists report a mechanism by which Twist recruits BRD4 to direct WNT5A expression in basal-like breast cancer (BLBC). Pharmacologic inhibition of the Twist-BRD4 association reduced WNT5A expression and suppressed invasion, cancer stem cell-like properties, and tumorigenicity of BLBC cells. [Cancer Cell] Abstract
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PUBLICATIONS (Ranked by impact factor of the journal)

EZH2 Expands Breast Stem Cells through Activation of NOTCH1 Signaling
Researchers identify an unrecognized Enhancer of Zeste Homolog 2 (EZH2)/NOTCH1 axis that controls breast tumor-initiating cells in triple-negative breast carcinomas. EZH2 overexpression increases NOTCH1 expression and signaling, and inhibition of NOTCH1 activity prevents EZH2-mediated stem cell expansion in nontumorigenic breast cells. [Proc Natl Acad Sci USA] Abstract

ß1 Integrin Inhibition Elicits a Prometastatic Switch through the TGFß-miR-200-ZEB Network in E-Cadherin-Positive Triple-Negative Breast Cancer
Researchers found that the loss of ß1 integrins attenuated breast tumor growth but markedly enhanced tumor cell dissemination to the lungs. When cultured in three-dimensional extracellular matrix scaffolds, antibodies that blocked ß1 integrin function or knockdown of ß1 switched the migratory behavior of human and mouse E-cadherin-positive triple-negative breast cancer cells from collective to single cell movement. [Sci Signal] Abstract

Intracellular Routing in Breast Cancer Cells of Streptavidin-Conjugated Trastuzumab Fab Fragments Linked to Biotinylated Doxorubicin-Functionalized Metal Chelating Polymers
To evaluate the cellular and nuclear uptake of trastuzumab Fab-streptavidin-biotin-metal-chelating polymer-doxorubicin (Dox), researchers incubated human epidermal growth factor receptor-2+ SK-BR-3 human breast cancer cells with the complexes saturated with stable In3+ and visualized their distribution by confocal fluorescence microscopy, monitoring the fluorescence of Dox. [Biomacromolecules]
Abstract | Graphical Abstract

Estradiol Stimulates Glucose Metabolism via 6-Phosphofructo-2-Kinase (PFKFB3)
Scientists demonstrated that PFKFB3 expression is highest in stage III lymph node metastases relative to normal breast tissues and that exposure of human MCF-7 breast cancer cells to estradiol causes a rapid increase in 14C-glucose uptake that is coincident with an induction of PFKFB3 mRNA, protein expression and the intracellular concentration of its product, fructose-2,6-bisphosphate. [J Biol Chem] Abstract

Differential ERa-Mediated Rapid Estrogenic Actions of Ginsenoside Rg1 and Estren in Human Breast Cancer MCF-7 Cells
Investigators addressed the differential abilities of Rg1 and estren in terms of the ability to activate estrogen receptor (ER) and the ability to induce ER translocation in MCF-7 cells. [J Steroid Biochem Mol Biol] Abstract

Cellular Responses of BRCA1-Defective and Triple-Negative Breast Cancer Cells and In Vitro BRCA1 Interactions Induced by Metallo-Intercalator Ruthenium(II) Complexes Containing Chloro-Substituted Phenylazopyridine
HCC1937 cells were significantly more sensitive to the ruthenium complexes than the MDA-MB-231 and MCF-7 cells. Treatment demonstrated a higher degree of cytotoxicity than cisplatin against all three cell lines. [BMC Cancer] Abstract | Full Article

GPER Functions as a Tumor Suppressor in MCF-7 and SK-BR-3 Breast Cancer Cells
The orphan, membrane-bound estrogen receptor (GPER)-specific agonist G-1 inhibited breast cancer cell proliferation in concentration-dependent manner via induction of the cell cycle arrest in M-phase, enhanced phosphorylation of histone 3 and cell apoptosis. [J Cancer Res Clin Oncol] Abstract

Human Sprouty1 Suppresses Growth, Migration, and Invasion in Human Breast Cancer Cells
Researchers demonstrated that in breast cancer cell lines, MDA-MB-231 and T47D, transfection-induced overexpression of human Sprouty1 reduced cell population, proliferation, and colony formation in vitro without affecting cell apoptosis. [Tumor Biol] Abstract


The PAM50 Risk-of-Recurrence Score Predicts Risk for Late Distant Recurrence after Endocrine Therapy in Postmenopausal Women with Endocrine-Responsive Early Breast Cancer
Researchers assessed the prognostic value of the PAM50 risk-of-recurrence score on late distant recurrence in a large cohort of postmenopausal, endocrine-responsive breast cancer patients. [Clin Cancer Res] Abstract

A Randomized Phase III Study Comparing Pegylated Liposomal Doxorubicin with Capecitabine as First-Line Chemotherapy in Elderly Patients with Metastatic Breast Cancer: Results of the OMEGA Study of the Dutch Breast Cancer Research Group BOOG
Both pegylated liposomal doxorubicin and capecitabine demonstrated comparable efficacy and acceptable tolerance as first-line single-agent chemotherapy in elderly patients with metastatic breast cancer, even in vulnerable patients or patients aged =75 years. [Ann Oncol] Abstract

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Aberrant DNA Methylation of MicroRNA Genes in Human Breast Cancer – A Critical Appraisal
The author discusses the publications describing aberrant methylation of microRNA genes in human breast cancer cells. Among the anti-tumorigenic properties of epigenetically inactivated microRNA genes, the inhibition of proliferation and of epithelial-to-mesenchymal transition are the best studied. [Cell Tissue Res] Abstract

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Generex Provides Update on Antigen Express Phase II AE37 Breast Cancer Vaccine Trial
Generex Biotechnology Corporation announced that recent results from a Phase II study of a novel cancer vaccine designed to prevent relapse in patients who have had breast cancer corroborate prior results from an interim analysis conducted in October of 2011. [Generex Biotechnology Corporation] Press Release

Syndax Announces NCI and FDA Reach Agreement on a Special Protocol Assessment (SPA) for the Phase III Trial of Entinostat in Patients with Advanced ER+ Breast Cancer
Syndax Pharmaceuticals, Inc. announced that the Division of Cancer Treatment and Diagnosis of the National Cancer Institute (NCI) has reached a special protocol assessment agreement with the U.S. Food and Drug Administration (FDA) for the planned pivotal Phase III trial of entinostat in patients with advanced breast cancer. [Syndax Pharmaceuticals, Inc.] Press Release

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NEW Tumor Microenvironment and Signaling
May 7-10, 2014
Heidelberg, Germany

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NEW PhD Position – Grading CIN in the Mammary Gland (European Molecular Biology Laboratory)

NEW Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

NEW Research Technologist – Particle Chemistry (STEMCELL Technologies Inc.)

NEW Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)

Studentship – Microtubules and End-Binding Proteins in Epithelial Remodeling and Breast Cancer Invasion (University of East Anglia)

Postdoctoral Fellow – Mechanisms that Contribute to Metastases of Breast Cancer Cells (Mount Sinai Medical Center/Icahn School of Medicine at Mount Sinai)

Postdoctoral Fellow – Hodgkin Lymphoma and Breast Cancer (Hackensack University Medical Center)

Postdoctoral Position – Functions of Epigenetic Enzymes in Breast Cancer (University of Wisconsin-Madison)

Postdoctoral Position – Breast Cancer Metastasis (Weizmann Institute of Science)

Postdoctoral Fellow – Morphogenetic Signaling Pathways in Development and Disease (University of Rochester Medical Center)

Postdoctoral Research Fellow (Brigham & Women’s Hospital and Harvard Medical School)

Postdoctoral Position – Stem Cells and Cancer (Brigham & Women’s Hospital – Harvard Medical School)

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